Extraction and screening of essential oils with high antibacterial and antifungal activities against pathogens of major crop of Gujarat.
Plant Disease Is Responsible For Significant Losses Of Global Crop Production Every Year And, Thus Has A Major Impact On The World’s Agricultural Productivity. The Control Of Bacterial And Fungal Diseases In Plants Is A Considerable Problem In Agriculture Practice. Many Phytopathogenic Bacteria And Fungi Have Acquired Resistance To Synthetic Pesticides. However, The Incessant And Indiscriminate Application Of These Chemical Pesticides Has Caused Health Hazards In Animals And Humans Due To Their Residual Toxicity. Spraying With Antibiotics, Fungicides, And Copper Compounds, Usually Suggested To Control Bacterial And Fungal Diseases, Has Never Been Satisfactory. Further Antibiotics And Fungicides Are Forbidden In Many Countries Because Of Their General Toxicity And Their Nature Of Exerting A Negative Impact On Both Yield And The Environment. There Is An Increasing Interest In The Use Of Alternative Antifungal And Antibacterial Agents That Are Nontoxic. One Such Alternative Is The Use Of Natural Plant Products. Relatively Limited Numbers Of Reports Are Found In The Literature Dealing With The Effect Of Essential Oils (EOs) And Their Components Against Many Pathogens. EOs Are The Subtle, Volatile Liquids Or Resins From Plants, Shrubs, Flowers, Wood, Roots, Bushes, And Seeds. Plant Volatiles Constitute About 1% Of Plant Secondary Metabolites And Are Mainly Represented By Terpenoids, Phenylpropanoids/benzeoids, Fatty Acid Derivatives, And Amino Acid Derivatives.
Objective of work:
To Isolate The Different Bacterial And Fungal Pathogen From The Major Crops Of Gujarat I.e. Cajanus Cajan, Arachis Hypogaea, Citrus Limon, Cicerari Etinum, Allium Sativum, Allium Cepa, Mangifera Indica The 100 EOs Were Evaluated For Their Antibacterial And Antifungal Effects Against Xanthomonas Campestris, Pantoea Agglomerans, Erwinia Chrysanthemi, Aspergill Usniger, Fusarium Oxysporum, Alternaria Porri And Colletotrichum Gloeosporioides Phytopathogens. According To The In Vitro Test Results, All Oils Showed More Or Less Antagonistic Activity Against All Tested Pathogen On Petri Plates Assays, Based On The Zone Of Inhibition. It Was Observed That All Tested Bacteria Fungi Were Highly Sensitive To The Essential Oils Of Cinnamon Species. From The Total Of 100 EOs, Nearly Seventeen EOs Showed Significant Activities In Almost All Organisms. The GC-MS Of These Seventeen EOs Was Accomplished To Investigate Their Chemical Nature. The Major Constituents Were Present In These Oils And It Was Inferred From Their Antibacterial Activities That They Act Synergistically. The Oil Of Cinnamomum Zeylanicum And Cinnamomum Cassia Were Most Potent Against All Bacteria. These Oil Constituted Cinnamaldehyde, Eugenol, And β – Linalool Which Might Be Responsible For Higher Activity Against The Tested All Pathogen.
Output of work
The Essential Oils Can Be Successfully Used As A Biocontrol Agent To Prevent The Growth Of All The Tested Pathogens. They May Be Cheaper Than The Total Crop Yield Loss. Our Results Indicate That These Essential Oils Will Be Successfully Applied As Potential Biocontrol Agents For The Management Of Diseases In A Variety Of Crops Caused By Different Phytopathogen. EOs Is Bio-efficacious, Economical, And Environmentally Safe And Can Be Ideal Candidates For Use As Agrochemicals.
Publications
V.C. Pawar And V.S. Thaker (2006) Efficacy Of 75 Essential Oils Against A. Niger. Mycoses 49:316-323 V.C. Pawar And V.S. Thaker (2007) Evaluation Of Anti Fusarium Oxysporum F. Sp Cicer And Anti Alternaria Porri Effect Of Some Essential Oils. World J Of Microbiol Biotech 23(8):1099-1106 M.H. Lodhia, K.R. Bhatt And V.S. Thaker (2009) Antibacterial Activity Of Essential Oils From Palmarosa, Evening Primrose, Lavender And Tuberose. Indian J Pharma Sci 71(2):131-134 K.S. Chudasama And V.S. Thaker (2012) Screening Of Potential Antimicrobial Compounds Against Xanthomonas Campestris From 100 Essential Oils Of Aromatic Plants Used In India: An Ecofriendly Approach. Arch Phytopathol Plant Prot 45(7):783-795 K.S. Chudasama And V.S. Thaker (2014) Biological Control Of Phytopathogenic Bacteria Pantoea Agglomerans And Erwinia Chrysanthemi Using 100 Essential Oils. Arch Phytopathol Plant Prot 1:1-12 V.P. Rabari, K.S. Chudasama And V.S. Thaker (2017) In Vitro Screening Of 75 Essential Oils Against Colletotrichum Gloeosporioides: A Causal Agent Of Anthracnose Disease Of Mango. International Journal Of Fruits Sciences.
Book chapter
Kiran S. Chudasama, Mital A. Vaghela, Dolly H. Parmar And Vrinda S. Thaker (2020). Antimicrobial Activity Of Some Plant Essential Oils Against Multidrug Resistant UTI Organism Accepted In “Advances In Essential Oils And Natural Products (volume 1). Akinik Publication, New Delhi, India. Pp.21-34 Kiran S. Chudasama, Jagdishchandra K. Monpara And Vrinda S. Thaker (2020). Plant Based Essential Oils, Its Components And Importance. Accepted In “Advances In Essential Oils And Natural Products (volume 1). Akinik Publication, New Delhi, India, Pp.1-19