This Project Is Focus On Standardizing The In Vitro Regeneration Protocol For Jamun.  Jamun Fruit Is Rich In Protein, Fiber, Antioxidants, Calcium, Iron, Phosphorous, Potassium, Manganese, Vitamin C And B6, And Several Other Nutrients. The Fruits Of Jamun Are Known To Possess Pharmaceutical And Therapeutic Properties. Fruits Are Used As An Effective Medicine Against Diabetes, Heart And Liver Troubles And Other Disease. Jamun Is Propagated Vegetatively By The Rooting Of Hardwood Cuttings, Grafting, And Budding Although The Establishment Of New Plants Requires One To Two Year. Micropropagation In Fruit Trees Would Help In Overcoming The Difficulties Of Vegetative Propagation, Producing True-to-type Plants, And Rapid And Mass Production Of Planting Materials. The Objective Of The Work Is To Standardize The In Vitro Regeneration Protocol For Jamun By Using Explant Embryos. The Embryo Was Cultured On MS Medium With Different Ratios NAA (0.1 To 0.5 Mg/L) And BAP (1.0 To 3.0 Mg/L), And IBA (0.5 To 1.5 Mg/L) Profound Growth Was Observed After Two Months Of Inoculation. The Best Result Was Initially Obtained With NAA (0.5mgL) And BAP 2mg/L) Ratio. The In Vitro Initiation Of The Culture Protocol Developed In This Experiment Could Profitably Be Explored For The Commercial Cultivation Of Syzygium Cumini.